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Description

Endocan is a proteoglycan composed of a 20 kDa mature polypeptide and a 30 kDa chondroitin /?dermatan sulfate chain.

Purified recombinant human Endocan (LIP-1001) is treated with proteinase K. Then the glycan chain is?purified by ion exchange on DEAE-sepharose

Key identified biological activities :

• increases endothelial migration induced by VEGF,
• comitogen with HGF / SF and FGF-2.

Characteristics

• Purified by ion exchange chromatography
• PM: 30kDa polyacrylamide gel revealed by Alcian blue
• Format: 10 μg
• When stored at -80 ° C in phosphate buffer without preservatives
• Applications: bioassays

Documentation

• Examples of protocols
• Béchard D, Gentina T, Delehedde M, Scherpereel A, Lyon M, Aumercier M, Vazeux R, Richet C, Degand P, Jude B, Janin A, Fernig DG, Tonnel AB, Lassalle P. Endocan is a novel chondroitin sulfate/dermatan sulfate proteoglycan that promotes hepatocyte growth factor/scatter factor mitogenic activity. J Biol Chem. 2001;276(51):48341-9
• Sarrazin S, Lyon M, Deakin JA, Guerrini M, Lassalle P, Delehedde M, Lortat-Jacob H. Characterization and binding activity of the chondroitin/dermatan sulfate chain from Endocan, a soluble endothelial proteoglycan. Glycobiology. 2010;20(11):1380-8

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